Separation, Characterization and Mammal Pancreatic Lipase Inhibitory Potential of Cucumber Flower Flavonoids

Edible flowers are a new “old” trend in modern gastronomy and healthy nutrition aimed at increasing food attractiveness improving the consumption of beneficial phytocompounds that have valuable properties. Cucumber (Cucumis sativus L., Cucurbitaceae family) is well-known agricultural product; it an important crop commonly used across globe. Despite sufficient knowledge nutrients secondary metabolites cucumber fruits, use for purposes still understudied. In this study, total flavonoid fraction was isolated purified after liquid–liquid solid-phase extraction polyamide Amberlite XAD-2 separation followed by high-performance liquid chromatography with photodiode array detection electrospray ionization triple quadrupole mass spectrometric (HPLC–PDA–ESI–tQ–MS/MS), resulting 47 compounds. Identified flavonoids included luteolin, apigenin, chrysoeriol-based flavones such as C-, O-, C,O-glycosides, most which were detected first time C. sativus. Flower extracts from ten cultivars quantified, isoorientin, isoorientin-4′-O-glucoside, cucumerins B D predominant. The vitro assay potential to inhibit mammalian pancreatic lipases demonstrated extracts. dominant compounds studied separately, luteolin derivatives determined be responsible bioactivity. To best our knowledge, study on has shown their wide dietary supplement.